DNA

Part:BBa_K1195009:Design

Designed by: Lindsey Perry, Xiuqi Li   Group: iGEM13_BYU_Provo   (2013-10-25)


T7 Bacteriophage Minor Capsid Protein (Wild Type)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 292
    Illegal BsaI.rc site found at 496


Design Notes

N/A


Source

We isolated the T7 bacteriophage genome by boiling phage for 5 minutes and then used PCR to amplify the minor capsid gene.

References